Ikram Tefiani1, Sidi Mohammed Lahbib Seddiki*1,2, Yassine Moustafa Mahdad2,3,Zahia Boucherit-Otmani1, Hidaya Fatima Zohra Touil1, Chahrazed Bessnouci2,4
1.LAPSAB Lab: Antifungal Antibiotic, Physico-Chemical Synthesis and Biological Activity, University of Tlemcen,Algeria.
2.University Center of Naâma, Algeria.
3.PPABIONUT Lab: Physiology, Physiopathology and Biochemistry of Nutrition, University of Tlemcen, Algeria.
4.Central medical biology laboratory, Public Hospital Establishment of Naâma, Algeria.
Candida albicans is the most frequently isolated opportunistic yeast in hospitals; consistently responsible for invasive fungal infections. The formation of biofilms and the activity of hy_drolytic enzymes are two major virulence factors contributing to the pathogenicity of this species. This study aimed to highlight the activity of hydrolytic enzymes in isolated strains of C. albicans which form biofilms, as well as the effect of the change in pH and temperature on their synthesis.The capacity to form biofilms was determined using the crystal violet technique. The synthesis of phospholipase was determined by the plate method using egg yolk culture medium. For the proteinase activity, agar plates containing bovine albumin serum was used. However, esterase, coagulase and hemolysin were evaluated using the opacity test, the conventional tube test and the sheep blood plaque test, respectively. In addition, phospholipase, proteinase and esterase activities were assessed under different conditions of temperature and pH. The isolated strains of C. albicans were able to form biofilms and synthesize phospholipase, proteinase, esterase, coagulase and hemolysin; the activities of these enzymes vary differently from one strain to another. C. albicans further exhibited hydrolytic activities. The interaction significance between the strains, pH and temperature depends on the type of enzymes. This draws attention to the importance of these enzymesto better understand the relationship be_tween the pathogenesis and the virulent process of this species.
Key words: Candida albicans, Biofilms, hydrolytic enzymes,temperature, pH_